Validation of MS/MS Data
Why do you need to validate search results?
The results of a database search are usually characterized by individual peptide/protein scores. The scores help to discriminate between correct and incorrect peptide assignments to spectra and therefore facilitate detection of false protein identifications. However, there are no universal criteria based on the database search scores for sorting out correct identifications from incorrect ones. Besides, while most of the database search engines apply similar methods to assign peptides from a sequence database to experimental MS/MS spectra, they all have different algorithms for calculating peptide scores. These scores are not equivalent and can not be compared directly.